FIELD: biotechnology.
SUBSTANCE: described is a method for indirect determination of titre of infectious activity of the causative agent of infectious feline rhinotracheitis in non-inactivated raw material for cultural vaccines using an amplification reaction of a target portion of the UL26.5-gene of viral DNA, involving the use of the developed oligonucleotides.
EFFECT: development of a highly sensitive, highly specific, fast, objective method not involving the use of a cell line for mediated determination of a titre of infectious activity of a causative agent of infectious feline rhinotracheitis in non-inactivated raw material for cultural vaccines by means of an amplification reaction of a target region of the UL26_5-gene of viral DNA.
1 cl, 2 dwg, 6 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS FHV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING POLYMERASE CHAIN REACTION AND QUANTITATIVE HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2808641C1 |
| METHOD FOR INDIRECT DETERMINATION OF INFECTIOUS ACTIVITY TITRE OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS IN RAW MATERIAL FOR VACCINE USING SECOND-ORDER DIFFERENTIAL OF POINT OF INTERSECTION OF LOGISTIC CURVE OF AMPLIFICATION REACTION OF UL35 GENE REGION | 2023 |
|
RU2823781C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "LAVR" OF INFECTIOUS FELINE RHINOTRACHEITIS CAUSATIVE AGENT IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS OF GENE UL47 USING DSSAFE FLUORESCENT DYE | 2024 |
|
RU2834233C1 |
| METHOD FOR INDIRECT DETERMINATION OF FELINE CALICIVIRUS INFECTIOUS ACTIVITY TITRE IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES BY MEANS OF QUANTITATIVE REGISTRATION OF REACTION OF AMPLIFICATION OF ORF1-GENE SECTION OF VIRAL RNA | 2023 |
|
RU2821027C1 |
| METHOD FOR DETERMINING TITRE OF INFECTIOUS ACTIVITY OF CANINE DISTEMPER AGENT IN RAW MATERIAL FOR MAKING CULTURE INACTIVATED VACCINES | 2024 |
|
RU2831062C1 |
| METHOD FOR INDIRECTLY DETERMINING THE TITER OF THE INFECTIOUS ACTIVITY OF FMDV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING A MATHEMATICAL DOUBLE DIFFERENTIAL OF THE CROSSING POINT DATA DURING AMPLIFICATION OF VIRAL CDNA | 2022 |
|
RU2793900C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2812858C1 |
| METHOD OF INDIRECT DETERMINING OF TITRE OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF CANINE DISTEMPER IN RAW MATERIALS FOR VACCINES USING METHOD OF REVERSE TRANSCRIPTION AND AMPLIFICATION REACTION IN REAL TIME | 2023 |
|
RU2815533C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2808585C1 |
| METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605 | 2024 |
|
RU2824661C1 |
