METHOD FOR ASSESSING INTESTINAL EPITHELIAL BARRIER PERMEABILITY IN PATIENTS RECEIVING HEMODIALYSIS TREATMENT Russian patent published in 2024 - IPC G01N33/66 G01N33/68 G01N33/573 G01N1/28 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to nephrology, gastroenterology, and can be used to assess intestinal epithelial barrier permeability in patients receiving haemodialysis treatment. In patients on an inter-dialysis day in the morning on an empty stomach, before taking medications, venous blood samples are taken in test tubes with a coagulation activator, which are left for 2 hours at room temperature, and then centrifuged at 3,000 rpm for 15 minutes. Concentration of D-lactate, mcmol/l, in the obtained serum is determined by immunoassay. Stool samples are analysed after preliminary sample preparation. Clinical material is added with 5 ml of a buffer, thoroughly suspended and centrifuged for 5 min/2,000 g. Formed supernatant of faeces samples is used for subsequent analysis immediately after its preparation. Concentration of zonulin, ng/ml, in faeces is determined by immunoassay, as well as concentration of calprotectin, mcg/g. Concentration value of each of the biomarkers is then evaluated in points from 0 to 3 depending on the concentration increase in accordance with table 2, the points are summed up and the permeability index is determined. If the permeability index is equal to 0, the absence of intestinal permeability disorders is determined, 1–3 – slightly increased intestinal permeability, 4–6 – moderately increased intestinal permeability, 7–9 – significantly increased intestinal permeability.

EFFECT: method provides the possibility of determining the inflammatory process in calculous pyelonephritis after performing percutaneous operations for removal of calculi in the postoperative period by using an electrochemical analysis of a urine sample, which enables to exclude false-positive results associated with change in colour of urine after percutaneous nephrolithotripsy, which do not allow determining blood corpuscles in a urine sample and accordingly diagnose.

1 cl, 7 tbl, 2 ex