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2 819 840

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IPC

C12Q1/6806(2018-01-01)

C12Q1/6827(2018-01-01)

C12Q1/686(2018-01-01)

(21) (22)

Application

2023134714, 2023-12-22

(24)

Start date

2023-12-22

(22)

Actual filing date

2023-12-22

(45)

Published

2024-05-27

(72)

Inventor

Bushueva Olga IurevnaPolonikov Aleksei ValerevichBykanova Marina Alekseevna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennyi Meditsinskii Universitet" Ministerstva Zdravookhraneniia Rossiiskoi Federatsii

METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs6677 (T>G) OF C11orf58 GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES Russian patent published in 2024 - IPC C12Q1/6806 C12Q1/6827 C12Q1/686

Abstract RU 2819840 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics, in particular, to a method for genotyping the polymorphic locus rs6677 (T>G) of the C11orf58 gene in a human by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5'-TTGTTATAGTAGAGCTGTTCATTATGG-3' and reverse 5'-TGTGAAAAGTACTTGCTCTCATGTT-3') and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5'-(FAM)CTTAGTTTCCTGTTACT(RTQ1)-3' and G-allele-specific fluorescent-labelled probe 5'-(ROX)CTTAGTTGCCTGTTACT(BHQ2)-3') in an amplifier with fluorescence detection.

EFFECT: invention widens the range of methods for genotyping polymorphic variants of the C11orf58 gene, is characterized by simplicity, accuracy and low cost.

1 cl, 1 dwg

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RU 2 819 840 C1

Authors

Bushueva Olga Iurevna

Polonikov Aleksei Valerevich

Bykanova Marina Alekseevna

Dates

2024-05-27—Published

2023-12-22—Filed