FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics, and can be used in medicine in determining hereditary predisposition to developing diseases associated with the carriage of the polymorphic variant rs11826990 (T>G) of the C11orf58 gene. Disclosed is a method for genotyping a polymorphic locus rs11826990 (T>G) of the C11orf58 gene in a human by real-time PCR using allele-specific fluorescent probes, providing for PCR using specially selected primers (forward 5'-CTGAGAGGGTCGAGGAGTGA-3' and reverse 5'-TCGACATTGTCACGGGAAC-3') and probes with fluorophores (A T-allele-specific fluorescent-labelled probe 5'-(FAM)TACTCAGTATAGAGAC(RTQ1)-3' and G-allele-specific fluorescent-labelled probe 5'-(ROX)TACTCAGGATAGAGAC(BHQ2)-3') in an amplifier with fluorescence detection.
EFFECT: invention widens the range of methods for genotyping polymorphic variants of the C11orf58 gene, is characterized by simplicity and accuracy.
1 cl, 1 dwg
