METHODS OF FORMING POLYPLEXES Russian patent published in 2024 - IPC A61K47/69 

FIELD: chemistry.

SUBSTANCE: present invention relates to a method of forming polyplexes based on nucleic acids, which can be used in gene therapy as safe and non-toxic transfection agents. Method includes several stages. At first, two liquid streams are created, one of which contains a polymer which is a highly branched poly(aminoether) (HPAE); and the second is a nucleic acid component. Polymer in the first liquid stream is then brought into contact with the nucleic acid component in the second liquid stream. Result is a polyplex having a size and a charge suitable for therapeutic administration. At the last stage, the obtained polyplex is isolated and stabilized.

EFFECT: proposed method is reproducible and scalable and also enables to obtain homogeneous and stable polyplexes.

43 cl, 1 dwg, 1 ex