FIELD: food industry.
SUBSTANCE: invention can be used to obtain soy protein hydrolyzate. A method of producing soy protein hydrolyzate by hydrolyzing soy protein isolate with an enzyme preparation of bacterial origin involves introducing into a thermostated reactor 10–17.5% of an aqueous suspension of soy protein isolate and an enzyme preparation obtained from Bacillus subtilis, added in an amount of 6–18 international units of activity per 1 grams of soy protein isolate. Then they are left in a reactor with a constantly operating rotor-stator dispersant at a temperature of 50°C without pH control for 1-2 hours, followed by inactivation of the enzyme by heating the reaction mixture to 68-70°C. The resulting product is then spray-dried in hot air or lyophilized. Preferably, the reaction mixture is exposed to a temperature of 68–70°C during enzyme inactivation for 10 minutes.
EFFECT: invention is aimed at developing a method of producing a mixture of peptides from plant protein using hydrolysis without the need to maintain pH and heat the reaction mixture to high temperatures, ensuring hydrolysis of 80% of the protein to peptides with a molecular weight of less than 26 kDa.
1 cl, 2 ex
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Authors
Dates
2024-06-28—Published
2021-11-12—Filed