METHOD OF PRODUCING PROTEIN FROM LEGUMINOUS CROPS Russian patent published in 2008 - IPC A23J1/14 A23J3/16 A23J3/34 C11B1/10 

FIELD: food products.

SUBSTANCE: method involves crushing plant raw material which is further subject to swelling in water. Then hydrolysis is carried out using microbially-derived enzyme preparations with their following inactivation. The obtained protein extract is centrifuged and ultrafiltrated. Then protein is precipitated in isoelectric point, neutralised and end product is dried. Enzyme preparations are neutral protease Bacillus subtilis with activity of 460-500 units/g in the amount of 0.01-0.03% to the raw material weight, cellulase Trichoderma reesei with activity of 7400-7600 units/g in the amount of 0.01-0.05% to the raw material weight, complex preparation produced by cultivation of bacteria Bacillus subtilis and fungi Penicillium emersonii and containing protease, amylase and β-glucanase with protease activity of 80-120 units/g in the amount of 0.01-0.015% to the raw material weight or mixture of them. Hydrolysis is carried out under the temperature of 50-70°C during 3-4 hours with the water duty of 1:8-1:10 and pH natural for the mixture of raw material and water. Additionally protein-fatty emulsion which is obtained at the extraction stage is splitted into protein and oil phases by centrifugation under 6000 rpm and 20°C and/or 0.3% NaCl in the form of 2.5% solution is added.

EFFECT: method improvement.

3 cl, 2 tbl, 5 ex