FIELD: chemistry.
SUBSTANCE: group of inventions relates to compounds and methods for detecting neutrophil elastase activity. Disclosed is a method for detecting neutrophil elastase activity in a tissue sample lysate, involving: (1) preparing a lysate from a tissue sample obtained from a subject; (2) contacting the lysate with a probe compound; (3) further subjecting at least an aliquot of the lysate obtained in step (2) to gel electrophoresis; (4) measurement of the detected signal. Also disclosed are a method for diagnosing the disease, probe compounds of formula I, formula II and formula IIA, as well as compositions for detecting neutrophil elastase activity and for diagnosing the disease.
EFFECT: group of inventions enables to analyse tissue lysate to determine activity of neutrophil elastase.
40 cl, 25 dwg, 1 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SULFOXONIUM YLIDE DERIVATIVES AS PROBES FOR CYSTEINE PROTEASE | 2019 |
|
RU2815018C2 |
| USING PROSOMATOSTATIN IN DIAGNOSTICS | 2012 |
|
RU2573985C2 |
| KIT FOR GIVING PRELIMINARY ACTION DIRECTION, METHOD AND AIDS USED THEREIN | 2010 |
|
RU2539915C2 |
| REPRESENTATIVE DIAGNOSIS | 2016 |
|
RU2743169C2 |
| ANTIBODIES SPECIFIC TO TUMOUR AND USE THEREOF | 2011 |
|
RU2595403C2 |
| SYNTHETIC LETHALITY AND TREATMENT OF CANCER | 2013 |
|
RU2676697C2 |
| SETS OF OLIGONUCLEOTIDE PROBES AND METHODS OF GASTROINTESTINAL MICROBIOTA PROFILING | 2011 |
|
RU2605913C2 |
| COMPOSITION AND METHOD FOR DETECTING MALIGNANT NEOPLASTIC DISEASE | 2015 |
|
RU2678135C1 |
| DETECTION METHOD OF INTESTINE INFLAMMATORY DISEASE | 2008 |
|
RU2502806C2 |
| TOM34 GENE RELATED TO COLON CANCER | 2006 |
|
RU2526196C2 |
