FIELD: veterinary virology.
SUBSTANCE: described is a method for molecular diagnostics, namely genotyping of vaccine strains of foot-and-mouth disease virus serotype SAT-2 by the method of studying peaks of melting points of amplicons using fluorescent dimeric acridine Eva488. For the first time, differentiation of genome of vaccine strains of foot-and-mouth disease virus serotype SAT-2 using the developed method is presented. Developed method is characterized by analytical specificity equal to 100%. In 95% confidence interval, the diagnostic sensitivity for the given method is 99.34–100.00%, the diagnostic specificity is 98.07–100.0%.
EFFECT: main advantage of the proposed invention is the ability to carry out in a short period of time, not more than 2.5 hours, genotyping of foot-and-mouth disease virus of serotype SAT-2 by the developed method.
2 cl, 2 dwg, 6 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DIFFERENTIATING GENOME OF FOOT-AND-MOUTH DISEASE VIRUS OF INDUSTRIAL STRAINS OF SEROTYPE A BY ANALYZING MAXIMUM MELTING POINTS OF PCR PRODUCTS USING INTERCALATING DYE SYBR GOLD | 2023 |
|
RU2823777C1 |
| CULTURE INACTIVATED ADSORBED VACCINE AGAINST FMD OF SAT-2/VII GENOTYPE FROM SAT-2/ERITREA/1998 STRAIN | 2023 |
|
RU2804875C1 |
| STRAIN OF FOOT AND MOUTH DISEASE VIRUS APHTAE EPIZOOTICAE FOR MANUFACTURING OF BIOLOGICAL PRODUCTS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FOOT AND MOUTH DISEASE OF SAT-2/VII GENOTYPE | 2023 |
|
RU2808493C1 |
| METHOD FOR DIFFERENTIATION OF GENOME OF ARRIAH VACCINE STRAIN FROM FIELD ISOLATES OF RABIES VIRUS BY POLYMERASE CHAIN REACTION IN REAL TIME WITH ANALYSIS OF PEAKS OF MELTING TEMPERATURES OF AMPLICONS AND USE OF ASYMMETRIC DYE SYBR GREEN | 2023 |
|
RU2822037C1 |
| STRAIN OF FOOT AND MOUTH DISEASE VIRUS APHTAE EPIZOOTICAE FOR MANUFACTURING OF BIOLOGICAL PRODUCTS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FOOT AND MOUTH DISEASE OF SAT-1/X GENOTYPE | 2023 |
|
RU2807038C1 |
| CULTURALLY INACTIVATED SORBED VACCINE AGAINST FOOT AND MOUTH DISEASE OF SAT-2/IV GENOTYPE | 2023 |
|
RU2815541C1 |
| TEST SYSTEM FOR ASSAY OF ANTIBODIES TO STRUCTURAL PROTEINS OF FOOT-AND-MOUTH DISEASE VIRUS GENOTYPE SAT-2/VII USING LIQUID-PHASE BLOCKING INDIRECT “SANDWICH” VERSION OF ELISA | 2023 |
|
RU2815531C1 |
| SAT-1/KENYA/2017 STRAIN OF APHTAE EPIZOOTICAE FOOT AND MOUTH DISEASE VIRUS FOR THE MANUFACTURE OF BIOLOGICAL PRODUCTS FOR THE DIAGNOSTICS AND SPECIFIC PREVENTION OF FOOT AND MOUTH DISEASE VIRUS OF SAT-1/NWZ GENOTYPE | 2023 |
|
RU2804634C1 |
| SAT-1/TANZANIA/2012 STRAIN OF APHTAE EPIZOOTICAE FOOT AND MOUTH DISEASE VIRUS SAT-1/I GENOTYPE FOR THE MANUFACTURE OF BIOLOGICAL PRODUCTS FOR THE DIAGNOSTICS AND SPECIFIC PREVENTION OF FOOT AND MOUTH DISEASE VIRUS SAT-1/I GENOTYPE | 2022 |
|
RU2799606C1 |
| CULTURE INACTIVATED EMULSION VACCINE AGAINST FOOT-AND-MOUTH DISEASE OF SAT-2/XIV GENOTYPE FROM SAT-2/XIV/2023 STRAIN | 2024 |
|
RU2824660C1 |
