FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, namely to a method for differentiating the genome of the foot-and-mouth disease virus of industrial strains of serotype O by analyzing the maximum point of Tm amplicons using the green intercalating dye YO-PRO-1 IODIDE. Method involves a polymerase chain reaction using specific oligonucleotide primers, a melting process of amplicons and determination of their melting point for differentiation of industrial strains of the serotype O foot-and-mouth disease virus.
EFFECT: developed method makes it possible: to increase sensitivity and specificity due to the use of highly specific original primers, calculated for the target portion of 1D gene of the foot-and-mouth disease virus serotype O, increase the accuracy of the analysis due to the use of the green intercalating dye YO-PRO-1 IODIDE, as well as by analyzing the maximum melting point (Tm – melting temperature) of amplicons to differentiate industrial strains of the serotype O foot-and-mouth disease virus.
2 cl, 3 dwg, 4 tbl, 3 ex
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| CULTURAL INACTIVATED EMULSION VACCINE AGAINST FOOT AND MOUTH DISEASE FROM O NO. 2620/ORENBURGSKY/2021 STRAIN OF O/ME-SA/IND-2001E GENOTYPE | 2023 |
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| CULTURAL INACTIVATED EMULSION VACCINE AGAINST O/EA-3 GENOTYPE FOOT-AND-MOUTH DISEASE OF O N2241/ETHIOPIA/2011 STRAIN | 2023 |
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| METHOD FOR RAPID QUANTITATIVE DETERMINATION OF THE LEVEL OF HUMORAL IMMUNITY OF ANIMALS TO STRAIN O N2311/ZABAIKALSKY/2016 OF GENOTYPE O/ME-SA/Ind-2001 OF FOOT AND MOUTH DISEASE VIRUS AFTER VACCINATION USING LIQUID-PHASE "SANDWICH" ELISA | 2022 |
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| O/KENYA/2017 STRAIN OF FMD VIRUS APHTAE EPIZOOTICAE GENOTYPE O/EA-2 FOR THE MANUFACTURE OF BIOLOGICAL PRODUCTS FOR THE DIAGNOSIS AND SPECIFIC PREVENTION OF FMD GENOTYPE O/EA-2 | 2022 |
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