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(19)

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2 825 470

(13)

(51)

IPC

C12N1/21(2006-01-01)

C12N9/12(2006-01-01)

C12N15/54(2006-01-01)

C12N15/70(2006-01-01)

(21) (22)

Application

2022133433, 2022-12-20

(24)

Start date

2022-12-20

(22)

Actual filing date

2022-12-20

(45)

Published

2024-08-26

(72)

Inventor

Nagornykh Maksim Olegovich

(73)

Holder

Avtonomnaya Nekommercheskaya Obrazovatelnaya Organizatsiya Vysshego Obrazovaniya Universitet

ESCHERICHIA COLI POLY(A)-POLYMERASE ENZYME PRODUCER STRAIN Russian patent published in 2024 - IPC C12N1/21 C12N9/12 C12N15/54 C12N15/70

Abstract RU 2825470 C2

FIELD: biotechnology.

SUBSTANCE: disclosed is a recombinant plasmid coding poly(A)-polymerase, and a recombinant bacterium Escherichia coli, which produces a poly(A)-polymerase enzyme encoded by SEQ ID NO: 4 and transformed by said recombinant plasmid. Also disclosed is use of said recombinant bacteria in in vitro synthesis of poly(A)-polymerase.

EFFECT: invention provides high expression of the target protein.

3 cl, 3 dwg, 2 ex

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RU 2 825 470 C2

Authors

Nagornykh Maksim Olegovich

Dates

2024-08-26—Published

2022-12-20—Filed