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2 817 377

(13)

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IPC

C12N15/82(2006-01-01)

C12Q1/6806(2018-01-01)

C12Q1/686(2018-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2023131563, 2023-12-01

(24)

Start date

2023-12-01

(22)

Actual filing date

2023-12-01

(45)

Published

2024-04-15

(72)

Inventor

Samarina Mariya AlekseevnaDivashuk Mikhail GeorgievichKarlov Gennadij IlichBazhenov Mikhail SergeevichKrupin Pavel YurevichArkhipov Andrej VladimirovichMiroshnichenko Dmitrij NikolaevichDolgov Sergej VladimirovichShulga Olga AlbertovnaKlementeva Anna AleksandrovnaTimerbaev Vadim Rafailovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Selskokhozyajstvennoj Biotekhnologii" Vniisb)

METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES Russian patent published in 2024 - IPC C12N15/82 C12Q1/6806 C12Q1/686 C12Q1/6876

Abstract RU 2817377 C1

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology and genetic engineering of plants, in particular to genetic markers detecting genetic modifications of the GBSSI gene, obtained using CRISPR/Cas9 on the genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on conducting a polymerase chain reaction (PCR) with oligonucleotide primers flanking the site of the intended editing, followed by detection of PCR results using capillary electrophoresis.

EFFECT: invention enables detecting GBSSI gene editing events in cereals resulting from editing using the CRISPR/Cas9 system.

3 cl, 7 dwg, 1 tbl, 2 ex

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RU 2 817 377 C1

Authors

Samarina Mariya Alekseevna

Divashuk Mikhail Georgievich

Karlov Gennadij Ilich

Bazhenov Mikhail Sergeevich

Krupin Pavel Yurevich

Arkhipov Andrej Vladimirovich

Miroshnichenko Dmitrij Nikolaevich

Dolgov Sergej Vladimirovich

Shulga Olga Albertovna

Klementeva Anna Aleksandrovna

Timerbaev Vadim Rafailovich

Dates

2024-04-15—Published

2023-12-01—Filed