FIELD: biotechnology.
SUBSTANCE: present invention relates to genetic engineering of plants, namely to genetic markers detecting genetic modifications of the ISA1 gene, obtained using CRISPR/Cas9 on genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on carrying out a polymerase chain reaction (PCR) with oligonucleotide primers, flanking an intended editing site, with subsequent detection of PCR results using capillary electrophoresis.
EFFECT: invention enables to detect ISA1 gene editing events in cereals arising from editing using the CRISPR/Cas9 system.
3 cl, 7 dwg, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2833966C1 |
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RU2833967C1 |
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|
RU2817377C1 |
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RU2817378C2 |
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RU2817384C2 |
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RU2800828C1 |
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RU2817376C2 |
