METHOD OF PRODUCING NANODISCS CONTAINING A β1-ADRENORECEPTOR IN A NATIVE CONFORMATION Russian patent published in 2024 - IPC A61K33/00 B82B1/00 B82Y5/00 

Abstract RU 2829120 C1

FIELD: biotechnology.

SUBSTANCE: described is a method of producing nanodiscs containing ADRB1 in native conformation. This method involves: preparing a membrane fraction from cells expressing ADRB1 on their surface; adding to the preparation a membrane fraction of cells of an amphipathic polymer selected from the group: Ultrasolute Amphipol 17, Ultrasolute Amphipol 18, AASTY 11–45, AASTY 11–50, AASTY 6–50, AASTY 6–55; centrifugation and sediment removal.

EFFECT: high efficiency of ADRB1 solubilization owing to use of amphipathic polymers.

1 cl, 1 dwg, 1 ex

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RU 2 829 120 C1

Authors

Peklo Mikhail Mikhajlovich

Lipatova Lyudmila Nikolaevna

Kashirina Natalya Mikhajlovna

Yanushevskaya Elena Vadimovna

Rutkevich Pavel Nikolaevich

Sharf Tatyana Vasilevna

Kuznetsova Irina Borisovna

Efremov Evgenij Evgenevich

Rybalkin Igor Nikolaevich

Vlasik Tatyana Nikolaevna

Dates

2024-10-24Published

2023-12-18Filed