FIELD: biochemistry; molecular biology.
SUBSTANCE: what is presented is a method for assessing the activity of a mammalian nucleotide excision repair (NER) system. Model DNA is obtained, which is a DNA duplex with size of 160 base pairs, both chains of which contain non-nucleoside inserts. Obtained model DNA is exposed to cell extracts containing NER proteins, which leads to removal of a volumetric damage in the composition of the adjacent DNA chain fragment. Chain integrity is restored by the presence of Taq-DNA polymerase and dNTP in the reaction mixture. Recovered DNA is amplified during subsequent PCR.
EFFECT: disclosed method has high sensitivity of the method, and also enables to reduce time spent on measurement.
1 cl, 3 dwg, 1 tbl, 2 ex
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