FIELD: molecular diagnostics.
SUBSTANCE: set of oligonucleotide primers and probes for the detection of African swine fever (ASF) virus DNA and bacteriophage lambda DNA as an internal control sample of the study are described. Also a method for detecting ASF virus DNA from biological material using a set of oligonucleotide primers and probes using loop isothermal amplification (LAMP) in the presence of an internal control sample of lambda bacteriophage DNA.
EFFECT: development of a highly sensitive, specific and rapid method for detecting African swine fever virus.
3 cl, 2 dwg, 6 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING DNA OF ASF VIRUS BY LOOP ISOTHERMAL AMPLIFICATION | 2018 |
|
RU2710065C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING RNA OF YELLOW FEVER VIRUS BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
|
RU2824209C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING DNA OF ORTHOPOXVIRUSES BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
|
RU2823776C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR IDENTIFYING RIFT VALLEY FEVER VIRUS RNA USING ISOTHERMAL PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2813519C1 |
| METHOD FOR DETECTING AFRICAN SWINE FEVER VIRUS DNA USING SPECIFIC OLIGONUCLEOTIDES BY ISOTHERMAL LOOP AMPLIFICATION AND COLORIMETRIC DETECTION OF AMPLIFICATION RESULTS | 2022 |
|
RU2806908C1 |
| OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT PROBE WITH INNER DAMPER, COMPLEMENTARY TO THE GENE SECTION P30 (CP204L) OF THE AFRICAN SWINE FEVER VIRUS, FOR USE IN POLYMERASE CHAIN REACTION IN REAL TIME | 2016 |
|
RU2606253C1 |
| METHOD FOR EDITING DNA OF AFRICAN SWINE FEVER VIRUS IN AREA OF GENES MGF 110_4L-6L USING PLASMIDS U6, pJET1.2 AND ENZYME Cas9 | 2023 |
|
RU2828403C1 |
| SET OF HIGHLY SPECIFIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETECTION AND DIFFERENTIATION OF ASF, CSF AND VD VIRUSES | 2024 |
|
RU2828887C1 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN BIOLOGICAL MATERIAL WITH A LOW VIRAL LOAD BASED ON TWO-STAGE PCR WITH DETECTION BY THREE TARGETS IN REAL TIME | 2020 |
|
RU2763173C1 |
| NUCLEIC ACID PROBE | 2014 |
|
RU2668154C2 |
