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2 799 410

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(51)

IPC

C12Q1/00(2006-01-01)

C12Q1/6876(2018-01-01)

C12Q1/6888(2018-01-01)

C07H21/00(2006-01-01)

C12N15/00(2006-01-01)

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Application

2022126146, 2022-10-06

(24)

Start date

2022-10-06

(22)

Actual filing date

2022-10-06

(45)

Published

2023-07-05

(72)

Inventor

Gnezdilova Larisa AleksandrovnaBorunova Saidfatima MirovnaDavydova Ekaterina EvgenevnaSelina Marina Viktorovna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennaia Akademiia Veterinarnoi Meditsiny I Biotekhnologii Mva Imeni K.I. Skriabina" Vo Mgavmib Mva Imeni K.I. Skriabina)

SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND A METHOD OF HIGHLY SENSITIVE DETECTION OF AFRICAN SWINE FEVER VIRUS DNA BY LOOP ISOTHERMAL AMPLIFICATION IN THE PRESENCE OF INTERNAL CONTROL SAMPLE DNA Russian patent published in 2023 - IPC C12Q1/00 C12Q1/6876 C12Q1/6888 C07H21/00 C12N15/00

Abstract RU 2799410 C1

FIELD: molecular diagnostics.

SUBSTANCE: set of oligonucleotide primers and probes for the detection of African swine fever (ASF) virus DNA and bacteriophage lambda DNA as an internal control sample of the study are described. Also a method for detecting ASF virus DNA from biological material using a set of oligonucleotide primers and probes using loop isothermal amplification (LAMP) in the presence of an internal control sample of lambda bacteriophage DNA.

EFFECT: development of a highly sensitive, specific and rapid method for detecting African swine fever virus.

3 cl, 2 dwg, 6 tbl, 3 ex

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RU 2 799 410 C1

Authors

Gnezdilova Larisa Aleksandrovna

Borunova Saidfatima Mirovna

Davydova Ekaterina Evgenevna

Selina Marina Viktorovna

Dates

2023-07-05—Published

2022-10-06—Filed