METHOD FOR ASSESSING ACTIVE FORMS OF OXYGEN IN DIAGNOSING MALE INFERTILITY Russian patent published in 2024 - IPC G01N33/50 A61B5/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, can be used in diagnosing spermatogenesis disorders and infertility and concerns a method for assessing reactive oxygen species (ROS) of spermatozoa. Ejaculate is collected; when preparing the ejaculate sample, it is chemically liquefied with the help of a liquefying solution. Prior to analysis, reaction cell with microgel is kept at temperature (+80…+100) °C until complete liquefaction of the microgel, the reaction cell is transferred to thermostat at 37 °C for 5 minutes to stabilize temperature. Efficiency of the microgel is determined by its colour, wherein reference results are used: a whitish-yellow colour of the melted gel at (+80…+100) °C is taken as a negative control and brought to room temperature microgel without addition of ejaculate; positive test corresponds to colour from light lilac to dark lilac melted with addition of 2–20 mcl of 2% H₂O₂ at (+80…+100) °C and maintained at 37 °C for 30 minutes of microgel. Prepared ejaculate sample in volume of 140 mcl is then added to the reaction cell with an effective microgel and stirred without formation of bubbles to reduce sperm cell stress. Ejaculate sample is mixed with the reaction cell microgel and held for 1–2 minutes. Further, the reaction cell is inserted into the opening of the floating plate at 37 °C, the cell is placed in thermostat at 37 °C for 30 minutes. ROS level is determined from the colour of the mixture of the ejaculate sample with the microgel as follows: with a white-yellow or light-pink colour of the mixture, a normal ROS level is determined, with a light lilac colour - a low ROS level, with a lilac colour - an average ROS level and with a dark colour, - lilac - about high level of ROS.

EFFECT: invention provides higher information value and reliability of diagnostics.

5 cl, 4 dwg, 1 ex