MUTANT ATP-DEPENDENT PROTEASE AND METHOD OF PRODUCING L-AMINO ACID USING SAME Russian patent published in 2024 - IPC C12N15/77 C12N9/50 C12P13/06 C12P19/08 

FIELD: biotechnology.

SUBSTANCE: disclosed is a polypeptide involved in the production of a branched-chain amino acid, containing deletion of amino acids corresponding to positions 431–433 based on amino acid sequence presented by SEQ ID NO: 5, where the branched chain amino acid is L-valine or L-isoleucine. Also disclosed is a microorganism of the genus Corynebacterium for producing said branched-chain amino acids, in which activity of ATP (adenosine triphosphate)-dependent protease is weakened in comparison with unmodified microorganism, wherein said microorganism contains said polypeptide or polynucleotide having a deletion of nucleotides corresponding to positions 1,291–1,299 based on the sequence presented by SEQ ID NO: 6. Also disclosed is a method of producing said branched-chain amino acids using said microorganism, as well as the use of a microorganism or polypeptide for producing said branched-chain amino acids.

EFFECT: invention enables to obtain L-valine or L-isoleucine with high output.

11 cl, 11 tbl, 4 ex