FIELD: biotechnology; forensic medicine; criminology.
SUBSTANCE: invention relates to the genetic research in the field of human identification. A method of obtaining molecular markers for determining the probable ethnogeographical origin of a person and a set of synthetic oligonucleotide primers for conducting multiplex polymerase chain reaction (PCR) are presented. The genotyping kit includes a composition formed by 55 pairs of synthetic original oligonucleotide primers for loci in the human genome, including SNP markers. Synthetic oligonucleotide primers are chosen in such a way that the PCR product has a minimum length of less than 150 bp. The invention also relates to a method of genotyping molecular markers to determine the probable ethno-geographical origin of a person by the method of multiplex amplification for working with samples of degraded DNA. The use of a method of obtaining molecular markers to determine the probable ethnogeographic origin of a person in the Russian population is proposed.
EFFECT: invention makes it possible to determine the probable ethnogeographical origin of a person when working with degraded DNA samples with high sensitivity, specificity and accuracy.
10 cl, 2 tbl, 1 ex
