Show metadata Hide metadata

(19)

(11)

2 839 687

(13)

(51)

IPC

C12N15/82(2006-01-01)

C12Q1/6806(2018-01-01)

C12Q1/686(2018-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2023131567, 2023-12-01

(24)

Start date

2023-12-01

(22)

Actual filing date

2023-12-01

(45)

Published

2025-05-07

(72)

Inventor

Samarina Mariya AlekseevnaDivashuk Mikhail GeorgievichKrupin Pavel YurevichKarlov Gennadij IlichBazhenov Mikhail SergeevichArkhipov Andrej VladimirovichMiroshnichenko Dmitrij NikolaevichDolgov Sergej VladimirovichShulga Olga AlbertovnaKlementeva Anna AleksandrovnaTimerbaev Vadim Rafailovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Selskokhozyajstvennoj Biotekhnologii" Vniisb)

METHOD FOR DETECTING SSIIA GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES Russian patent published in 2025 - IPC C12N15/82 C12Q1/6806 C12Q1/686 C12Q1/6876

Abstract RU 2839687 C1

FIELD: biotechnology.

SUBSTANCE: present invention relates to genetic engineering of plants, namely to genetic markers detecting genetic modifications of the SSIIA gene, obtained using CRISPR/Cas9 on genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on carrying out a polymerase chain reaction (PCR) with oligonucleotide primers, flanking an intended editing site, with subsequent detection of PCR results using capillary electrophoresis.

EFFECT: invention enables detecting SSIIA gene editing events in cereals resulting from editing using the CRISPR/Cas9 system.

3 cl, 5 dwg, 1 tbl, 2 ex

Similar patents RU2839687C1

Title	Year	Author	Number
METHOD FOR DETECTING RSR1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2833967C1
METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2834229C1
METHOD FOR DETECTING SBEIIA GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2833965C1
METHOD FOR DETECTING ISA1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2833963C1
METHOD FOR DETECTING ISA1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2833966C1
METHOD FOR DETECTING RSR1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Krupin Pavel Yurevich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2833964C1
METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES	2023	Samarina Mariya Alekseevna Divashuk Mikhail Georgievich Karlov Gennadij Ilich Bazhenov Mikhail Sergeevich Krupin Pavel Yurevich Arkhipov Andrej Vladimirovich Miroshnichenko Dmitrij Nikolaevich Dolgov Sergej Vladimirovich Shulga Olga Albertovna Klementeva Anna Aleksandrovna Timerbaev Vadim Rafailovich	RU2817377C1
MOLECULE OF RNA-CONDUCTOR SGRNA FOR INTRODUCING MUTATIONS INTO CONSERVED REGION OF COMMON WHEAT PPD-D1 GENE PROMOTER REGION USING CRISPR/CAS9 GENOME EDITING SYSTEM	2024	Kiseleva Antonina Andreevna Timonova Ekaterina Mikhajlovna Kolozhvari Anastasiya Eduardovna Salina Elena Artemovna	RU2822358C1
METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD	2022	Blinkov Andrej Olegovich Divashuk Mikhail Georgievich Zlobin Nikolaj Evgenevich Ivanova Lyubov Aleksandrovna Komakhin Roman Aleksandrovich Konovalova Lyudmila Nikolaevna Lebedeva Marina Valerevna Taranov Vasilij Vasilevich Ulyanov Daniil Sergeevich	RU2817376C2
METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY	2022	Blinkov Andrej Olegovich Divashuk Mikhail Georgievich Zlobin Nikolaj Evgenevich Ivanova Lyubov Aleksandrovna Komakhin Roman Aleksandrovich Konovalova Lyudmila Nikolaevna Lebedeva Marina Valerevna Taranov Vasilij Vasilevich Kharchenko Petr Nikolaevich	RU2817384C2

RU 2 839 687 C1

Authors

Samarina Mariya Alekseevna

Divashuk Mikhail Georgievich

Krupin Pavel Yurevich

Karlov Gennadij Ilich

Bazhenov Mikhail Sergeevich

Arkhipov Andrej Vladimirovich

Miroshnichenko Dmitrij Nikolaevich

Dolgov Sergej Vladimirovich

Shulga Olga Albertovna

Klementeva Anna Aleksandrovna

Timerbaev Vadim Rafailovich

Dates

2025-05-07—Published

2023-12-01—Filed