FIELD: biotechnology.
SUBSTANCE: invention relates to genetic engineering of plants, namely to genetic markers detecting genetic modifications of the ISA1 gene, obtained using CRISPR/Cas9 on genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on carrying out a polymerase chain reaction with oligonucleotide primers, flanking the site of the putative editing, with subsequent detection of PCR results using capillary electrophoresis.
EFFECT: invention provides a simpler method for detecting changes in nucleotide sequences of target genes, in particular the ISA1 gene, arising in the genome as a result of editing using the CR1SPR/Cas9 system.
3 cl, 5 dwg, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING ISA1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
|
RU2833963C1 |
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|
RU2833965C1 |
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|
RU2833967C1 |
| METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
|
RU2834229C1 |
| METHOD FOR DETECTING RSR1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
|
RU2833964C1 |
| METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
|
RU2817377C1 |
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817383C2 |
| RNA-CONDUCTOR MOLECULE FOR GENOMIC EDITING OF PROMOTER REGION OF VRN-A1 GENE OF MONOCOTYLEDONOUS CEREALS USING CRISPR/CAS9 SYSTEM | 2020 |
|
RU2762831C1 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817378C2 |
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817384C2 |
