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2 833 965

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IPC

C12N1/20(2006-01-01)

C12Q1/6806(2018-01-01)

C12Q1/686(2018-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2023131568, 2023-12-01

(24)

Start date

2023-12-01

(22)

Actual filing date

2023-12-01

(45)

Published

2025-02-03

(72)

Inventor

Samarina Mariya AlekseevnaDivashuk Mikhail GeorgievichKrupin Pavel YurevichKarlov Gennadij IlichBazhenov Mikhail SergeevichArkhipov Andrej VladimirovichMiroshnichenko Dmitrij NikolaevichDolgov Sergej VladimirovichShulga Olga AlbertovnaKlementeva Anna AleksandrovnaTimerbaev Vadim Rafailovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Selskokhozyajstvennoj Biotekhnologii" Vniisb)

METHOD FOR DETECTING SBEIIA GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES Russian patent published in 2025 - IPC C12N1/20 C12Q1/6806 C12Q1/686 C12Q1/6876

Abstract RU 2833965 C1

FIELD: biotechnology.

SUBSTANCE: present invention relates to genetic engineering of plants, namely to genetic markers detecting genetic modifications of SBEIIA gene, obtained using CRISPR/Cas9 on genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on carrying out a polymerase chain reaction (PCR) with oligonucleotide primers, flanking an intended editing site, with subsequent detection of PCR results using capillary electrophoresis.

EFFECT: invention enables detecting SBEIIA gene editing events in cereals resulting from editing using the CRISPR/Cas9 system.

3 cl, 5 dwg, 1 tbl, 2 ex

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RU 2 833 965 C1

Authors

Samarina Mariya Alekseevna

Divashuk Mikhail Georgievich

Krupin Pavel Yurevich

Karlov Gennadij Ilich

Bazhenov Mikhail Sergeevich

Arkhipov Andrej Vladimirovich

Miroshnichenko Dmitrij Nikolaevich

Dolgov Sergej Vladimirovich

Shulga Olga Albertovna

Klementeva Anna Aleksandrovna

Timerbaev Vadim Rafailovich

Dates

2025-02-03—Published

2023-12-01—Filed