FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for constructing a display vector of genes of antigen-specific binding polypeptides. Method comprises processing by using a restriction endonuclease specifically recognizing a restriction site to obtain four nucleic acid fragments, having specific sticky ends, and then enabling directed ligation of nucleic acid fragments.
EFFECT: method can be used for efficient screening of antigen-specific antigen-binding polypeptides or their fragments.
24 cl, 14 dwg, 4 tbl, 10 ex
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