FIELD: biotechnology; molecular biology.
SUBSTANCE: disclosed is a method of isolating double-stranded RNA from the strain of E. coli HT115, transformed by the plasmid L4440, which carries the insertion of the target fragment. Bacterial suspension is lysed. Lysate is treated with DNase. Desired dsRNA is extracted using a mixture of methanol and chloroform. Target dsRNA is then precipitated in 7.5% PEG-8000 in presence of 0.5 M NaCl. Traces of the mixture are removed by washing with ethanol. Obtained dsRNA fraction is dissolved in deionised water.
EFFECT: invention provides reducing the number of operations for producing a preparation of exogenously synthesized dsRNA.
1 cl, 3 dwg, 1 ex
