FIELD: biotechnology.
SUBSTANCE: described is a method for genotyping a polymorphic locus rs547025 (T>C) of the SIRT3 gene in a human by real-time PCR, involving the use of selected primers and fluorescent-labelled probes.
EFFECT: development of easy-to-implement method of genotyping of single-nucleotide polymorphism rs547025 (T>C), localized in position chr11:232855 (GRCh38_p14) of the SIRT3 (Gene ID: 23410) gene by real-time polymerase chain reaction using allele-specific signalling probes containing FAM and ROX fluorophores.
1 cl, 1 dwg
Authors
Dates
2025-02-24—Published
2024-06-25—Filed