FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics, and can be used in medicine in determining hereditary predisposition to developing diseases associated with the carriage of the polymorphic variant rs13161158 (T>C) of the HSPA4 gene. Disclosed is a method for genotyping a polymorphic locus rs13161158 (T>C) of the HSPA4 gene in a human by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5'-CCAGTGGCTGATACACCAGA-3' and reverse 5'-GGAAGACACTTACGCATTCCA-3') and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5'-(FAM)CCATCTAGTGCCCCTAGCT(RTQ1)-3' and C-allele-specific fluorescent-labelled probe 5'-(ROX) CCATCTAGCGCCCCTAGCT(BHQ2)-3') in an amplifier with fluorescence detection.
EFFECT: invention widens the range of methods for genotyping polymorphic variants of the HSPA4 gene, is characterized by simplicity and accuracy.
1 cl, 1 dwg
