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2 830 244

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IPC

C12Q1/6806(2018-01-01)

C12Q1/6827(2018-01-01)

C12Q1/686(2018-01-01)

(21) (22)

Application

2024115497, 2024-06-06

(24)

Start date

2024-06-06

(22)

Actual filing date

2024-06-06

(45)

Published

2024-11-18

(72)

Inventor

Bushueva Olga Iurevna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennyi Meditsinskii Universitet" Ministerstva Zdravookhraneniia Rossiiskoi Federatsii

METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs4279640 (T>C) OF HSF1 GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES Russian patent published in 2024 - IPC C12Q1/6806 C12Q1/6827 C12Q1/686

Abstract RU 2830244 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to a method for genotyping a polymorphic locus rs4279640 (T>C) of the HSF1 gene in humans. Method is carried out by means of a real-time polymerase chain reaction and involves the use of forward and reverse primers with sequences of SEQ ID NO: 1 and 2 respectively, as well as rs4279640-T- and C-allele-specific fluorescent-labelled probes with sequences SEQ ID NO: 3 and 4 respectively.

EFFECT: present invention provides a simple and cost-effective method of genotyping single nucleotide polymorphism rs4279640 (T>C).

1 cl, 1 dwg

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RU 2 830 244 C1

Authors

Bushueva Olga Iurevna

Dates

2024-11-18—Published

2024-06-06—Filed