FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, molecular genetic diagnostics and can be used in medicine in determining the hereditary predisposition to the development of diseases associated with the carriage of the polymorphic variant rs910652 (T>C) of the HSPA12B gene. Disclosed is a method for genotyping a polymorphic variant rs910652 (T>C) of the HSPA12B gene in a human by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5′-ATCTCAAGCCTGCCTGTGG-3′ and reverse 5′-CCGTCCTGATCTTTGCACAG-3′) and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5′-(FAM)CTACACTTTTCACTGGAAG(RTQ1)-3′ and C-allele-specific fluorescent-labelled probe 5′-(ROX)CTACACTTCTCACTGGAAG(BHQ2)-3′) in an amplifier with fluorescence detection.
EFFECT: invention widens the range of methods for genotyping polymorphic variants of the HSPA12B gene, is characterized by simplicity, accuracy and low cost.
1 cl, 1 dwg
