FIELD: biotechnology.
SUBSTANCE: present invention relates to the field of biotechnology, in particular to systems for controlling gene expression, systems for creating synthetic genetic circuits in living cells, systems for targeted delivery of molecules to regions of the genome, genome and epigenome editing systems based on nucleotide sequence coding nCas9/dCas9 proteins, and nucleotide sequences coding RNA molecules with self-dimerizing RNA domains, as a tool for increasing the binding specificity of CRISPR/nCas9 and CRISPR/dCas9 with target DNA sequences.
EFFECT: method for increasing the binding specificity of CRISPR/nCas9 and CRISPR/dCas9 complexes, according to the present invention, is of great importance for creating cellular biomedical products and gene therapy of human hereditary diseases.
6 cl, 7 dwg, 1 ex
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