FIELD: biotechnology.
SUBSTANCE: following is presented: a nucleotide sequence-based editing system encoding SuCas9_NLS protein as a tool for introducing targeted double-strand breaks in the DNA of an eukaryotic cell for editing the human genome, an expression vector and a method for editing the eukaryotic cell genomic DNA sequence.
EFFECT: genome editing system according to the invention is of great importance for gene therapy of human hereditary diseases.
6 cl, 4 dwg, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DNA-CUTTING AGENT BASED ON Cas9 PROTEIN FROM THE BACTERIUM STREPTOCOCCUS UBERIS NCTC3858 | 2022 |
|
RU2788197C1 |
| DNA POLYMER BASED ON Cas9 PROTEIN FROM BIOTECHNOLOGICALLY SIGNIFICANT BACTERIUM CLOSTRIDIUM CELLULOLYTICUM | 2018 |
|
RU2712497C1 |
| DNA PROTEASE CUTTING AGENT BASED ON Cas9 PROTEIN FROM DEFLUVIIMONAS SP. | 2018 |
|
RU2712492C1 |
| NUCLEASE Cpf1 FROM BACTERIUM RUMINOCOCCUS BROMII, DNA MOLECULE OR RNA CODING NUCLEASE, VECTOR CONTAINING SAID DNA MOLECULE, CRISPR/Cpf1 SYSTEM CONTAINING SAID NUCLEASE AND GUIDE RNA, HOST CELL FOR PRODUCING NUCLEASE Cpf1, METHOD OF PRODUCING NUCLEASE Cpf1 AND USE THEREOF | 2022 |
|
RU2816876C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
|
RU2722934C1 |
| USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS | 2019 |
|
RU2724470C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM DEMEQUINA SEDIMINICOLA BACTERIA | 2019 |
|
RU2722933C1 |
| DNA CUTTER BASED ON THE ScCas12a PROTEIN FROM THE BACTERIUM SEDIMENTISPHAERA CYANOBACTERIORUM | 2022 |
|
RU2791447C1 |
| DNA-CUTTING AGENT BASED ON THE Cas9 PROTEIN FROM THE BACTERIUM CAPNOCYTOPHAGA OCHRACEA | 2021 |
|
RU2778156C1 |
| TOOL FOR CUTTING DOUBLE-STRANDED DNA USING CAS12D PROTEIN FROM KATANOBACTERIA AND HYBRID RNA PRODUCED BY FUSION OF GUIDE CRISPR RNA AND SCOUT RNA | 2020 |
|
RU2771626C1 |
