FIELD: biotechnology.
SUBSTANCE: method for determining the concentration of feline calicivirus virions in raw material for whole-virus vaccines, comprising recovering feline calicivirus RNA by a solid-phase method; performing a reverse transcription reaction and amplification of a target region of the viral cDNA ORF1 gene using oligonucleotides: FCV-F3145 with design 5'-TGGCAAAGGTGGCGTCAAAC-3', FCV-P3194 (FAM/GTQ1) with design 5'-GCTGTGGATCGTGGTTATGACG-3', FCV-R3238 with design 5'-TGTGGAACTTGGTGCCTGGT-3'. Oligonucleotides are designed for the target region of the feline calicivirus ORF1 gene in range of 3145-3257 bp for amplification of fragment with size of 113 bp; determining a differential of the second degree for a CP point during amplification of a target region of the FCV ORF1 gene, calculating the concentration of FCV virions using a mathematical formula.
EFFECT: developed method makes it possible to increase specificity, increase sensitivity and increase reliability of the performed analysis.
2 cl, 5 dwg, 4 tbl, 4 ex
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