FIELD: molecular biology.
SUBSTANCE: what is described is a method for differentiation of the strain “Mira” of feline calicivirus from the other strains of calicivirus by analysing single-nucleotide polymorphisms of ORF1 gene. Method involves using oligonucleotide primers designed for amplifying a PCR product sized 93 bp, and SYBR Green dye, and then analysing single-nucleotide polymorphisms of ORF1 gene.
EFFECT: developed method enables higher sensitivity, specificity and reliability of the analysis.
2 cl, 5 dwg, 5 tbl, 3 ex
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RU2826458C1 |
