METHOD FOR DETECTING ALLERGEN-SPECIFIC ANTIBODIES USING SOLID-STATE MICROCHIPS OF ALLERGENS Russian patent published in 2025 - IPC G01N33/53 

FIELD: medical science.

SUBSTANCE: invention refers to detection of human allergen-specific antibodies, particularly to increasing sensitivity and specificity of detection of human allergen-specific antibodies by means of allergen microchips. Disclosed is a method which involves detection of allergen-specific antibodies using two fluorescent probes, wherein the first probe consists of a biological recognition molecule one conjugated with an organic fluorescent dye and the second probe consists of a biological recognition molecule two conjugated with a fluorescent quantum dot, wherein the biological recognition molecule one and the biological recognition molecule two bind the allergen-specific antibody at two different epitopes, and the maximum of the fluorescence spectrum of the quantum dot spectrally overlaps with the maximum of the absorption spectrum of the organic fluorescent dye, wherein the solid-state microchip comprises a substrate that is located in an area where allergens to which allergen-specific antibodies bind are immobilized, has a Bragg mirror, which reflection spectrum overlaps with the quantum dot fluorescence spectrum and the organic fluorescent dye absorption spectrum, in addition, the quantum dots excitation is carried out by the light source in the quantum dots absorption region, and detection of the fluorescent signal is carried out at the fluorescence wavelength of the organic fluorescent dye excited by the quantum dots according to the Förster resonant energy transfer mechanism.

EFFECT: higher sensitivity of allergen-specific antibody detection by means of solid-state allergen microchips that enables diagnosing early allergic sensitization.

4 cl, 2 dwg, 1 ex