FIELD: biotechnology, medicine, biochemistry.
SUBSTANCE: group of inventions relates to analytical biochemistry and immunochemical analysis. Biological microchip for the detection of exosomes in human serum, which is an array of three-dimensional hemispherical hydrogel elements 100 mcm in diameter on the surface of a glass slide, is disclosed, hydrogel elements are obtained by polymerizing a mixture containing methacrylamide-based gel-forming monomers and CD81, CD147, A33 monoclonal antibodies, while the biological microchip includes hydrogel elements, in each of which an individual antibody is immobilized, as well as empty hydrogel elements that do not contain immobilized molecular probes, to control non-specific interactions. Method for detecting exosomes in human serum using said biological microchip is also disclosed.
EFFECT: group of inventions allows detecting exosomes in human serum for the purpose of diagnosing colorectal cancer.
2 cl, 4 dwg, 3 ex
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| METHOD OF TREATMENT OF BLOOD SAMPLE AT IMMUNOLOGICAL ANALYSIS OF ALLERGEN-SPECIFIC AND TOTAL IMMUNOGLOBULINS E | 2012 |
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RU2816512C1 |
| METHOD FOR DETECTING ANTIBODIES - CLASS G IMMUNOGLOBULINS IN BLOOD SERUM TO PATHOGENS OF SEVERE ACUTE RESPIRATORY VIRAL INFECTIONS, INCLUDING SARS-COV-2, WITH SIMULTANEOUS PROGNOSIS OF COVID-19 SEVERITY, BASED ON HYDROGEL BIOCHIP | 2020 |
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