METHOD OF PRODUCING ANTIGENS OF CELL WALLS OF GRAM-POSITIVE COCCI Russian patent published in 2025 - IPC C12N1/20 A61K39/85 

FIELD: biotechnology.

SUBSTANCE: disclosed is a method of producing antigens from bacterial cell walls, comprising adding to cells of gram-positive cocci from the genera Staphylococcus and Streptococcus in amount of 1010 cells in an isotonic solution containing 30% mannitol, 10 mM MES with pH 6.5, 1 mM EDTA, 1 mM PMSF, oligochitosan with a weight-average molecular weight of 5 kDa in amount of 1 mcg; then the mixture is incubated for 15 minutes at 37 °C, bacterial protoplasts are separated by centrifugation at 2500 g for 5 minutes, the obtained antigen solution is dialyzed, concentrated and lyophilized.

EFFECT: invention provides a wider range of methods for producing antigens from cell walls of staphylococci and streptococci without using protein enzymes.

1 cl, 3 ex