METHOD OF SYNTHESIS OF DYE FOR ENZYME ACTIVITY ASSAY Russian patent published in 1994 - IPC

FIELD: organic chemistry, dyes. SUBSTANCE: synthesis of dye is carried out by azo coupling of derivative of naphthylamine and diazonium salt of the general formula I and II , where R - mono- or dialkylamino-group, piperidyl, morpholyl; group [R-N=N^⊖] represents: a) ; b) ; c) ; d) ; e) ; f) . Y-BE⊖4

; Cl^⊖·1.2 ZnCl₂; 1/2SO⊖4. New compounds enable to enhance the precision of trypsin activity assay by 8.3-times as compared with 1-(carbobenzoxyarginyl)-aminonaphthalene-5-sulfoacid due to enhanced sensitivity of dyes. They improve also kinetic properties of chromogenic substrates in the process of serine proteases assay as compared with known substrate. EFFECT: improved method of enzyme activity assay. 4 tbl