HYBRID STRAIN OF ANIMAL MUS MUSCULUS CELLS FOR PRODUCING MONOCLONAL ANTIBODIES AGAINST HUMAN IgG Russian patent published in 1994 - IPC

Abstract RU 2008350 C1

FIELD: biochemical technology. SUBSTANCE: hybrid strain of animal Mus musculus cells which is producer of anti-human IgG monoclonal antibodies is produced by hybridization of IgG antigen-pulsed spleen macrophages of Balb 1c mice with myelocytes Sp 2/0 Ag. 14. Monoclonal antibodies is anti-human IgG 2a antibodies having the capacity of site-specific binding of human IgG-type molecules except their Fab-Fc fragments. The titers of such monoclonal antibodies are of 5-10 μg/ml in liquid culture medium and about 5-10 mg/ml in ascite liquid. EFFECT: increased antibody-producing capacity. 1 tbl

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RU 2 008 350 C1

Authors

Efetov K.A.

Tehtin S.Ju.

Knjazeva O.A.

Dates

1994-02-28—Published

1992-01-23—Filed