FIELD: biotechnology, virology. SUBSTANCE: invention relates to preparing the strain of hybrid cultured murine cells HBSV-4 producing monoclonal antibodies to a-determinant of hepatitis B virus surface antigen. Strain is obtained by hybridization of myeloma cells P3-NS1-Ag 4-1 with murine splenocytes of strain BALB/c immunized with hepatitis B virus surface antigen isolated from serum blood of patients with chronic hepatitis. The obtained strain produces monoclonal antibodies of class IgG1 interacting with conformational epitope on group-specific a-determinant HBSAg and showing high stability. Monoclonal antibodies ensure to detect HBSAg in solutions and biological fluids of organism. Invention can be used for hepatitis B diagnosis. EFFECT: strain indicated above. 1 dwg
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