FIELD: virology. SUBSTANCE: hybridoma is prepared by fusion of myeloma murine cells (strain PAJ) with murine splenocytes (strain BALB/c) preliminary immunized with 146S-component of foot and mouth disease virus Asia-1 (strain N 48). On the media of mammalian cell cultures (DMEM, IMDM) at 37 C hybridoma produces monoclonal antibodies to foot and mouth disease virus Asia-1 in cultural fluid. Antibody titer in cultural fluid determined by indirect IFA method is 10-210-3. Hybridoma administrated into abdominal cavity of mice (strain BALB/c) induces the formation of ascite liquid containing monoclonal antibodies showing titer in IFA 10-210-3, in PH 4.66 log2, in P3 3.0log10. Strain is denoted at No. IIA4- 90(GF Asia-1-90/11 and deposited at number VSKK(II) No. 517 D. EFFECT: preparing of hybrid strain indicated above. 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS MUS MUSCULUS L , PRODUCING MONOCLONAL ANTIBODIES TO 146 S-COMPONENT OF FOOT-AND-MOUTH VIRUS A@@@ (AGLIE) | 0 |
|
SU1751202A1 |
| STRAIN OF HYBRID CULTURED CELLS OF ANIMAL MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODIES RAISED TO PIG VESICULAR DISEASE VIRUS, STRAIN T-75 | 1997 |
|
RU2117700C1 |
| STRAIN OF THE HYBRID CULTURED ANIMAL CELL MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODY RAISED TO AUJESZKY'S DISEASE VIRUS, STRAIN UNIIEV-18B | 1995 |
|
RU2092554C1 |
| FOOT-AND-MOUTH VIRUS STRAIN = 1737/GEORGIA/2000 OF TYPE ASIA-1 FOR PREPARING DIAGNOSTIC AND VACCINE PREPARATIONS | 2002 |
|
RU2218397C1 |
| ASIA-1-TYPE FOOT-AND-MOUTH DISEASE VIRUS STRAIN FOR PRODUCING OF DIAGNOSTIC AND/OR VACCINE PREPARATIONS | 2005 |
|
RU2297452C2 |
| METHOD OF PREPARING SERUM FOR SERODIAGNOSIS OF FOOT AND MOUTH DISEASE TYPE A | 1999 |
|
RU2140290C1 |
| TEST SYSTEM FOR DETERMINING TITER OF ANTIBODIES AGAINST STRUCTURAL PROTEINS OF ASIA-1 N 2356/14/2018 FOOT-AND-MOUTH DISEASE VIRUS STRAIN USING LIQUID-PHASE BLOCKING INDIRECT "SANDWICH" VERSION OF ELISA | 2023 |
|
RU2812211C1 |
| FOOT AND MOUTH DISEASE VIRUS STRAIN A(GEORGIA)1999/ 1721 TYPE A FOR PREPARING DIAGNOSTIC AND VACCINE PREPARATIONS | 2003 |
|
RU2242513C1 |
| TEST SYSTEM FOR QUANTITATIVE DETECTION OF ANTIBODIES AGAINST 146S COMPONENT OF FOOT-AND-MOUTH DISEASE VIRUS GENOTYPE O/EA-3 IN ANIMAL BLOOD SERUM BASED ON LIQUID-PHASE ENZYME IMMUNOASSAY | 2023 |
|
RU2817382C1 |
| STRAIN OF THE HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODIES TO A-DETERMINANT OF HEPATITIS B VIRUS SURFACE ANTIGEN | 1994 |
|
RU2097426C1 |
