STRAIN OF BACTERIUM ACINETOBACTER CALCOACETICUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-GGTACC-3' Russian patent published in 1995 - IPC

Abstract RU 2034922 C1

FIELD: microbiology and biotechnology. SUBSTANCE: method involves detection of strain-producer Acinetobacter calcoaceticus ВКПМ B-6337. Strain is non-pathogenic, it provides the preparing of restriction endonuclease which recognizes and splits the nucleotide sequence 5'-GGTACC-3' with high yield and activity of enzyme. Enzyme yield is 60 000 U/g wet biomass, specific activity of the end enzyme is 10 000-100 000 U/ml. Restriction endonuclease Acc 651 is an isoschizomer of enzyme Kpn I and can replace the latter in all genetic engineering works. EFFECT: increased yield of the end product. 1 dwg

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RU 2 034 922 C1

Authors

Repin V.E.

Degtjarev S.Kh.

Rechkunova N.I.

Dates

1995-05-10—Published

1993-03-24—Filed