STRAIN OF BACTERIUM DELEYA AQUAMARINA - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-GTGCAC-3' Russian patent published in 1998 - IPC

Abstract RU 2105811 C1

FIELD: biotechnology, biochemistry, genetic engineering. SUBSTANCE: strain Deleya aquamarina - a producer of restriction endonuclease DagI is isolated from sea-water as a result of search and screening restrictase producers. Strain provides preparing restrictase that recognizes and cleaves nucleotide sequence 5'-GTGCAC-3'. Enzyme yield is 18000 U/g crude biomass, specific activity of the end enzyme is 20000 U/ml. Restictase DagI is an isoschizomer of restrictase 5′-GTGCAC-3′ and can replace the latter in all genetic-engineering works. Invention can be used for research of primary DNA structure and mapping of different genomes. EFFECT: strain-producer indicated above, increased activity and yield of enzyme. 1 dwg

Similar patents RU2105811C1

Title	Year	Author	Number
STRAIN OF BACTERIUM STREPTOMYCES SPECIES SJ-12 - PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-CTGCAG-3'	1997	Puchkova L.I. Andreeva I.S. Ushakova T.A. Krivopalova G.N. Repin V.E.	RU2135581C1
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GGTNACC-3'	1996	Andreeva I.S. Puchkova L.I. Saranina I.V. Lokhova I.A. Repin V.E.	RU2115728C1
BACTERIUM STRAIN SPHINGOBACTERIUM MIZUTAE-32 AS PRODUCER OF SPML RESTRICTION ENDONUCLEASE, RECOGNIZING AND CLEAVING 5'-AT'CGAT-3' NUCLEOTIDE SEQUENCE	2003	Puchkova L.I. Radionenko Ju.V. Ushakova T.A. Repin V.E.	RU2266323C2
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS 22 AS PRODUCER OF SITE-SPECIFIC RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-CCNNNNN^NNGG-3' AND ELICITING GROWTH-STIMULATING ACTIVITY WITH RESPECT TO CULTURAL PLANT SEEDS	2002	Puchkova L.I. Andreeva I.S. Ushakova T.A. Radionenko Ju.V. Repin V.E. Polushkina A.F.	RU2238972C2
METHOD OF ENDONUCLEASE RESTRICTION BSE 21 I PREPARING	1999	Mikhajlova V.K. Puchkova L.I. Kuvshinov V.N. Ushakova T.A. Repin V.E.	RU2184777C2
BACTERIAL STRAIN OF Arthrobacter species Mn372 AS PRODUCER OF Asi3721 RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING OF 5'-ATGCAT-3' NUCLEOTIDE SEQUENCE	2005	Puchkova Larisa Ivanovna Totmenina Ol'Ga Dmitrievna Afonina Veronika Sergeevna Polushkina Alla Fridrikhovna Repin Vladimir Evgen'Evich	RU2302459C2
METHOD OF RESTRICTION ENDONUCLEASE PRODUCING	1997	Puchkova L.I. Mikhajlova V.K.	RU2136749C1
STRAIN OF BACTERIUM Paenibacillus sp, PRODUCER OF RESTRICTION ENDONUCLEASE Psp 1009I	2007	Puchkova Larisa Ivanovna Andreeva Irina Sergeevna Saranina Irina Vasil'Evna Pechurkina Natal'Ja Ivanovna Afonina Veronika Sergeevna Repin Vladimir Evgen'Evich	RU2340663C1
STRAIN OF BACTERIUM PHOTOBACTERIUM PHOSPHOREUM - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GG(T/C)(AG)CC-3'	1994	Repin V.E. Puchkova L.I. Rodicheva Eh.K. Vydrjakova G.A.	RU2110573C1
STRAIN OF BACTERIUM BACILLUS PUMILIS PRODUCING RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′- CCTNAGC- 3′	1992	Degtjarev S.Kh. Rechkunova N.I. Repin V.E.	RU2021373C1

RU 2 105 811 C1

Authors

Repin V.E.

Puchkova L.I.

Ushakova T.A.

Mikhajlova V.K.

Repina M.V.

Litosh V.G.

Dates

1998-02-27—Published

1996-05-06—Filed