STRAIN OF BACTERIUM BACILLUS BADIUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-(A/T)CCGG(A/T)-3′ Russian patent published in 1996 - IPC

Abstract RU 2053299 C1

FIELD: microbiology, biotechnology. SUBSTANCE: strain of bacterium Bacillus badius ВКМП B-6616 provides preparing restrictase Bba 179 I which recognizes and splits the nucleotide sequence 5′-(A/T)CCGG(A/T)-3′,. Strain does not show demanding to nutrient media, cultivation period is short. Strain B. badius ВКМП B-6616 is isolated from the soil as a result of the search of restrictase producers. The yield of enzyme is 1000 U/g crude biomass, specific activity of the end enzyme is 3000 U/ml. Restrictase Bba 179 I is an isoschizomer of restrictase Bet I. EFFECT: preparing restriction endonuclease indicated above, increased yield of enzyme. 1 dwg

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RU 2 053 299 C1

Authors

Degtjarev S.Kh.

Repin V.E.

Rechkunova N.I.

Shevchenko A.V.

Abdurashitov M.A.

Dates

1996-01-27—Published

1993-12-22—Filed