FIELD: medicine, virology. SUBSTANCE: virus of Venezuelan equine encephalomyelitis, strain Trinidad, GKV number 536, is multiplied in human embryo diploid lung cells L-68 at 37 C for 18-24 h. Virus is concentrated with polyethylene glycol 6000, treated by ultrasonic oscillation (3 times by 15 s). Virus-containing material is purified in sucrose gradient density, inactivated with 0.05% formaldehyde for 48 h and sorbed on aluminium hydroxide at the ratio 0.8-1.2/8-12 at pH 7,3 ± 0,1 Final protein concentration in vaccine is 100 ± 10 mcg/ml, activity is 3.3-3.9 lg PFU. EFFECT: improved method of vaccine preparing. 3 cl, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING THE INACTIVATED VACCINE FOR PROPHYLAXIS OF VENEZUELAN ENCEPHALOMYELITIS | 1987 |
|
RU2078816C1 |
| STRAIN OF RECOMBINANT SMALL POX VIRUS EXPRESSING STRUCTURAL PROTEINS OF THE HORSE VENEZUELAN ENCEPHALOMYELITIS VIRUS AND USEFUL FOR IMMUNOBIOLOGICAL PREPARATIONS PRODUCTION AND A METHOD OF ITS PREPARING | 1993 |
|
RU2091489C1 |
| VACCINE AND METHOD FOR INCREASING IMMUNOGENICITY AND SPECIFIC ACTIVITY OF INFECTION PREVENTION AGENTS | 2020 |
|
RU2741834C1 |
| METHOD OF PREPARING INACTIVATED VACCINE AGAINST CLASSIC PLAGUE IN PIGS | 1993 |
|
RU2049477C1 |
| PREPARATION COMPRISING LIQUID IMMUNOGLOBULIN AGAINST EQUINE VENEZUELAN ENCEPHALOMYELITIS FROM HORSE BLOOD SERUM (EQUINE IMMUNOGLOBULIN EVE) | 2003 |
|
RU2261113C2 |
| METHOD FOR PRODUCTION OF IMMUNOGENIC PREPARATIONS USING PROTEASOME SYSTEM USEFUL IN SCREENING OF AGENTS FOR VIRAL INFECTION PROPHYLAXIS | 2004 |
|
RU2279290C2 |
| METHOD OF PREPARING VACCINE AGAINST HEPATITIS A | 1989 |
|
SU1672635A1 |
| METHOD FOR PREPARING COMBINED BIVALENT, TISSUE-CULTURE, INACTIVATED, CONCENTRATED, PURIFIED VACCINE FOR PREVENTING HEMORRHAGIC FEVER AND RENAL SYNDROME | 2009 |
|
RU2445117C2 |
| METHOD OF PREPARING ANTIRABIC INACTIVATED CULTURAL VACCINE | 1994 |
|
RU2077338C1 |
| METHOD FOR PREPARING INACTIVATED VACCINE AGAINST HEPATITIS A VIRUS | 2006 |
|
RU2314125C1 |
