STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF SITE-SPECIFIC ENDONUCLEASE WHICH RECOGNIZES AND SPLITS THE NUCLEOTIDE SEQUENCE 5′-CTCTTC-3′ Russian patent published in 1996 - IPC

Abstract RU 2054040 C1

FIELD: microbiology, biotechnology. SUBSTANCE: method involves the detection of strain-producer providing the preparing thermostable site-specific endonuclease which recognizes and splits the nucleotide sequence 5′-CTCTTC-3′. The new strain Bacillus coagulans ВКПМ B-6309 (116) is proposed - a producer of restriction endonuclease Bco116 I isolated from thermal sources as a result of restriction endonuclease search. The yield of enzyme is 8000 U/g wet biomass, specific activity of the end enzyme is 10000 U/ml. Restrictase Bco116 I is an isoschizomer of restrictase Bco632 I and can replace the latter in all genetic engineering studies. EFFECT: detection of new strain-producer of enzyme, high yield of enzyme.

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RU 2 054 040 C1

Authors

Repin V.E.

Tereshchenko T.A.

Lebedev L.R.

Dates

1996-02-10—Published

1993-01-22—Filed