FIELD: biotechnology. SUBSTANCE: recombinant surface antigen of hepatitis B is prepared by cultivation of yeast strain-producer on the nutrient medium consisting of pepton and yeast extract and containing 10-200 mg/l inorganic phosphate followed by incubation of antigen on the nutrient medium containing 5-50 mg/l inorganic phosphate. EFFECT: improved method of antigen preparing.
Authors
Dates
1995-09-27—Published
1991-12-27—Filed