FIELD: medicinal biotechnology, genetic engineering, virology. SUBSTANCE: invention relates to preparing the recombinant plasmid DNA pDES 20 encoding synthesis of hepatitis B virus surface antigen (HBsAg/ayw), the yeast strain Saccharomyces cerevisiae DAN-041/pDES 20 producing HBsAg/ayw and vaccine based on thereof. Constructed recombinant plasmid DNA pDES 20 has gene HBsAg/ayw under control of the modified promoter region of the yeast gene PH05 that is able to independent replication in E. coli and S. cerevisiae cells. Strain S. cerevisiae DAN-041/pDES 20 is obtained at yeast cells transformation with recombinant plasmid pDES 20. Strain produces antigen HBsAg/ayw at expression level up to 5 mg/1 l culture and showing 100% stability of plasmid under nonselective growth conditions. Vaccine for hepatitis B control contains an effective amount of hepatitis B virus recombinant surface antigen (HBsAg/ayw), adjuvant, preserving agent, physiologically acceptable dilutant and shows high immunogenicity at minimal by-side reactive effect. EFFECT: recombinant plasmid DNA indicated above, high expression level of antigen. 3 cl
