FIELD: biotechnology. SUBSTANCE: method involves cultivation of strain being producer of yeast followed by incubation of antigen. Said cultivation is carried out on culture medium which contains peptone, yeast concentrate and 40-200 mg/l of inorganic phosphorous. Mentioned above incubation takes place on culture medium which contains 5-50 mg/l of inorganic phosphorous. Additionally medium which contains peptone, glucose and yeast extract at weight ratio 0.05-0.15:1:0.05-0.15 is also fed. EFFECT: improves efficiency of the method.
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Authors
Dates
1995-10-10—Published
1991-12-27—Filed