FIELD: genetic engineering, molecular biology, microbiology.
SUBSTANCE: invention can be used for the development of microbiological yeast producer providing high yield of recombinant antigen of hepatitis virus B of serotype adw. By carrying out genetic-engineering procedures the yeast strain Hansenula polymorpha VKPM Y-2412 is prepared that is transformed with DNA sequence encoding HBsAg/adw fused with gene MOX promoter and with gene TRP3 and representing the producer of recombinant surface antigen of hepatitis B virus. Invention allows preparing enhanced levels in synthesis of antigen and simplifying methods for preparing cultures of high density.
EFFECT: valuable properties of yeast strain.
8 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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| MIXED VACCINE FOR IMMUNOPROPHYLAXIS OF VIRAL HEPATITIS B AND D, TETANUS AND DIPHTHERIA | 2003 |
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|
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|
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| RECOMBINANT PLASMID DNA PHBS PROVIDING BIOSYNTHESIS OF SURFACE ANTIGEN OF HEPATITIS B VIRUS, METHOD OF CONSTRUCTION OF PLASMID, YEAST STRAIN PICHIA PASTORIS PS103 (PHBS) AS PRODUCER OF INDICATED ANTIGEN | 2000 |
|
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| RECOMBINANT Hansenula polymorpha YEAST STRAIN - PRODUCER OF MUTANT HEPATITIS B VIRUS SURFACE ANTIGEN (VERSIONS) | 2015 |
|
RU2586513C1 |
| RECOMBINANT Hansenula polymorpha YEAST STRAIN - PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN SEROTYPE "ayw" | 2015 |
|
RU2586511C1 |
| RECOMBINANT PLASMID ENCODING HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG), ITS PREPARING AND YEAST STRAIN HANSENULA POLYMORPHA AS PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG) | 2002 |
|
RU2207374C1 |
| RECOMBINANT STRAIN OF YEAST HANSENULA POLYMORPHA - PRODUCENT OF CAPSID PROTEIN OF HEPATITIS E VIRUS | 2012 |
|
RU2493249C1 |
