FRAGMENT OF DOMEN OF ALPHA-GLOBULIN GENES IN CHICKEN Russian patent published in 1995 - IPC

FIELD: biotechnology. SUBSTANCE: genetic engineering construction is created based on the DNA fragment (size is 1737 nucleotide pairs) from domen of alpha-globin gene in chicken. Construction has the attachment site of DNA to nuclear matrix, tissue-specific transcription enhancer, the site of replication onset and constitutive site of hypersensitivity to DNase I. Domen fragment is located at distance 4000 nucleotide pairs from chicken alpha-pi globin genes. For preparing transgenic animals (rabbit, sheep) original genetic engineering construction pMTBGHa is created containing proposed fragment of domen of chicken alpha-globin genes. The presence of this construction in animal genome with investigated fragment provided the increase of gene BGH expression. The level of BGH expression in transgenic rabbit with proposed DNA fragment in matured age was an average 55 ng/ml as compared with 7.0 ng/ml in rabbit no containing indicated fragment. Invention can be used scientific investigations and for cell strains and transgenic animals preparing as producers of pharmacologically valuable substances. EFFECT: preparing fragment indicated above. 5 dwg, 5 tbl