FIELD: medicine. SUBSTANCE: method involves freezing rapidly extracted pieces of fresh tissues to -30 C on cooled block holder of cryostat. 15-20 mcm thick cryostatic slices are produced, placed on cover slides and placed into 0.4-0.5% orthophthalic aldehyde solution in 0.1 N hydrochloric acid on boiling water bath. The slices are dried in air jet during 15 min, enclosed in light mineral oil and examined using fluorescent microscope. EFFECT: enhanced effectiveness of cytological and histological analysis of parenchymal cells of pineal gland and isolated cells of diffuse endocrine system, producing hormones.
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